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KMID : 1161420110140030181
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Journal of Medicinal Food
2011 Volume.14 No. 3 p.181 ~ p.194
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Effects of Acute Oral Administration of Vitamin C on the Mouse Liver Transcriptome
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Jun Hee-Jin
Kim Su-Kyung
Dawson Kevin
Choi Dal-Woong
Kim Jong-Sang
Rodriguez Raymond L.
Lee Sung-Joon
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Abstract
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Vitamin C is a strong antioxidant that alters gene expression in cells, and its effects can be modified by cellular oxidative stress. We investigated the genome-wide effects of vitamin C on the in vivo transcriptome in the liver, which synthesizes various enzymes and proteins to defend against cellular oxidative stress. We fed mice vitamin C (0.056?mg/g of body weight) for 1 week and performed DNA microarray analysis with hepatic mRNA in fasting and refeeding states to mimic physiological conditions of oxidative stress. Significance analysis of microarray data identified approximately 6,000 genes differentially expressed in both fasting and refeeding states. In the fasting state, vitamin C induced overall energy metabolism as well as radical scavenging pathways. These were ameliorated in the refeeding state. These findings suggest that vitamin C has profound and immediate global effects on hepatic gene expression, which may help prevent oxidative stress, and that long-term treatment with vitamin C might reduce the risk of chronic disease.
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KEYWORD
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antioxidant activity, liver, microarray, nutrigenomics, real-time polymerase chain reaction, vitamin C
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